Cellufine Sulfate

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Cellufine Sulfate
For concentration, purification and depyrogenation of virus, viral/microbial antigens and heparin-binding proteins.

Advances in vaccines and clinical diagnostics have created an increasing demand for large volumes of highly purified and concentrated virus and viral or microbial antigens. Cellufine Sulfate affinity media is a simple, rapid and effective means for concentration, purification and depyrogenation of these important products.

Cellufine Sulfate eliminates cumbersome, time-consuming and potentially unsafe classical ultra-centrifugation and density gradient methods. It can also provide a significant improvement in concentration and purity. Cellufine Sulfate can reduce or eliminate the expense, ligand leakage and reproducibility problems associated with immobilized dextran sulfate, chondroitin sulfate or heparin.
Elution of the bound product is affected through simple stepwise or gradient increases in ionic strength.

Features:

• Affinity for a wide range of live, killed or disrupted viruses, viral or microbial antigens and heparin-binding proteins
• Closed column operation assures safety and product sterility
• Endotoxins do not bind, allowing a rapid and contaminant free depyrogenation
• Rigid, high-strength beads
• Autoclavable

Benefits:

• More effective than ultracentrifugation at removing contaminants from culture media and host cells
• Avoids excessive product handling and safety concerns, particularly with viral preparations
• Simultaneous concentration and purification improve yield, reduce processing steps, time and costs
• Gentle binding and elution conditions provide high capacity and product yield
• Resists compression, providing rapid flow for high-speed processing, even in large columns, making it easily scalable
• Resistant to chemical depyrogenation with base and chemically sterilizable with formalin